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Circularization of genes and chromosome by CRISPR in human cells

2018-04-19

Abstract excerpt

Extrachromosomal circular DNA (eccDNA) and ring chromosomes are genetic alterations found in humans with genetic disorders and diseases such as cancer. However, there is a lack of genetic engineering tool to recapitulate these features. Here, we report the discovery that delivery of pairs of CRISPR/Cas9 guide RNAs into human cells generate functional eccDNAs and ring chromosomes. We generated a dual-fluorescence e...

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Literature Corpus work
8f8836fd-47d5-5fd1-8e96-2f9721c64d11
DOI
10.1101/304493
Open publication

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Circularization of genes and chromosome by CRISPR in human cellsDOI 10.1101/304493
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