Article
Expanding the editable genome and CRISPR-Cas9 versatility using DNA cutting-free gene targeting based on in trans paired nicking.
Nucleic acids research - 24 Jan 2020
Chen Xiaoyu, Tasca Francesca, Wang Qian, Liu Jin, Janssen Josephine M, Brescia Marcella D, Bellin Milena, Szuhai Karoly, Kenrick Josefin, Frock Richard L, Gonçalves Manuel A F V
Abstract excerpt
Genome editing typically involves recombination between donor nucleic acids and acceptor genomic sequences subjected to double-stranded DNA breaks (DSBs) made by programmable nucleases (e.g. CRISPR-Cas9). Yet, nucleases yield off-target mutations and, most pervasively, unpredictable target allele disruptions. Remarkably, to date, the untoward phenotypic consequences of disrupting allelic and non-allelic (e.g....
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