Article

Molecular mechanism of plasmid elimination by the DdmDE defense system

2024-05-10

Abstract excerpt

Seventh pandemic Vibrio cholerae strains contain two hallmark pathogenicity islands that encode the DNA defense modules DdmABC and DdmDE. Here we use cryo-EM to reveal the mechanistic basis for plasmid defense by DdmDE. A cryo-EM structure of the DdmD helicase-nuclease reveals that it adopts an auto-inhibited dimeric architecture. The prokaryotic Argonaute protein DdmE uses a DNA guide to target plasmid DNA. A st...

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Literature Corpus work: 8803184e-2948-5533-b087-711497fc99dc
DOI: 10.1101/2024.05.10.593530

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