Article

Mn <sup>2+</sup> -Induced Structural Flexibility Enhances the Entire Catalytic Cycle and the Cleavage of Mismatches in Prokaryotic Argonaute Proteins

2023-06-29

Abstract excerpt

Prokaryotic Argonaute (pAgo) proteins, a class of DNA/RNA-guided programmable endonucleases, have been extensively utilized in nucleic acid biosensors. The specific binding and cleavage of nucleic acids by pAgo proteins, which are crucial processes for their applications, are dependent on the presence of Mn 2+ bound in the pockets, as verified through X-ray crystallography. However, a comprehensive understanding...

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Literature Corpus work: 5597f715-008e-5e98-851e-d76c07859ce8
DOI: 10.1101/2023.06.28.546964

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