Article

Single-Cell and Tissue-Specific CRISPR Editing Analyses Unveil New Insights to Off-Targets and Translocations

2026-04-15

Abstract excerpt

CRISPR-Cas9 holds promise for treating genetic disease, but rare off-target mutations and structural variants remain as key safety concerns, especially at scales relevant to therapy. We established workflows to resolve Cas9 off-target activity in vitro at single-cell resolution and in vivo across different tissues. Using clonally expanded electroporated mouse embryos and embryonic stem cells, we reveal that indivi...

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Literature Corpus work: 7edf78d6-9c9e-50ce-a9bd-de049373c8cf
DOI: 10.64898/2026.04.13.718115

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