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Article

OliTag-seq enhances in cellulo detection of CRISPR-Cas9 off-targets.

Communications biology - 6 Jun 2024

Yang Zhi-Xue, Deng Dong-Hao, Gao Zhu-Ying, Zhang Zhi-Kang, Fu Ya-Wen, Wen Wei, Zhang Feng, Li Xiang, Li Hua-Yu, Zhang Jian-Ping, Zhang Xiao-Bing

Abstract excerpt

The potential for off-target mutations is a critical concern for the therapeutic application of CRISPR-Cas9 gene editing. Current detection methodologies, such as GUIDE-seq, exhibit limitations in oligonucleotide integration efficiency and sensitivity, which could hinder their utility in clinical settings. To address these issues, we introduce OliTag-seq, an in-cellulo assay specifically engineered to enhance the...

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Identifiers

PMID: 38844522
DOI: 10.1038/s42003-024-06360-w
PMCID: PMC11156888

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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