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Genome-wide detection of CRISPR editing in vivo using GUIDE-tag

21 Jan 2022

Abstract excerpt

Analysis of off-target editing is an important aspect of the development of safe nuclease-based genome editing therapeutics. in vivo assessment of nuclease off-target activity has primarily been indirect (based on discovery in vitro, in cells or via computational prediction) or through ChIP-based detection of double-strand break (DSB) DNA repair factors, which can be cumbersome. Herein we describe GUIDE-tag,...

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PMID: 35064134
DOI: 10.1038/s41467-022-28135-9

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