Article

Baculovirus-vectored precision delivery of large DNA cargoes in human genomes

2020-08-17

Abstract excerpt

Precise gene editing and genome engineering by CRISPR technology requires simultaneous delivery of multiple DNA-encoded components into living cells rapidly exceeding the cargo capacity of currently utilized viral vector systems. Here we exploit the unmatched heterologous DNA cargo capacity of baculovirus to resolve this bottleneck. We implement hybrid DNA techniques (MultiMate) for rapid and error-free assembly o...

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Literature Corpus work: 7c5a026a-2e6c-5186-aca2-aff141df5398
DOI: 10.1101/2020.08.17.253898

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