Article

Versatile and efficient non-viral integration of large transgenes in human T cells via CRISPR knock-in and engineered integrases

2025-09-11

Abstract excerpt

Current gene transfer methods often lack the precision, versatility, or efficiency when integrating large transgenes, limiting the ability to engineer therapeutic T-cells with more complex payloads. Here, we report ‘one-pot’ PASTA (Programmable and Site-specific Transgene Addition), a non-viral genome engineering strategy for large gene insertion that combines CRISPR-Cas-mediated homology-directed repair (HDR) and...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 8f6564e8-cd1e-55cc-8cc9-70be7c6605d6
DOI: 10.1101/2025.09.10.675267

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Versatile and efficient non-viral integration of large transgenes in human T cells via CRISPR knock-in and engineered integrasesDOI 10.1101/2025.09.10.675267Read

High-efficiency nonviral CRISPR/Cas9-mediated gene editing of human T cells using plasmid donor DNASemantic proximity Read

CRISPR-Mediated Non-Viral Site-Specific Gene Integration and Expression in T Cells: Protocol and Application for T-Cell TherapySemantic proximity Read

Efficient nonviral integration of large transgenes into human T cells using Cas9-CLIPTSemantic proximity Read

Ultra-large targeted DNA integrations in primary human cellsSemantic proximity Read

High-efficiency targeted transgene integration via primed micro-homologuesSemantic proximity Read

Orthogonal CRISPR systems for targeted integration and multiplex base editing enable nonviral engineering of allogeneic CAR-T cellsSemantic proximity Read

Current Non-Viral-Based Strategies to Manufacture CAR-T CellsSemantic proximity Read

Self-cutting and integrating CRISPR plasmids (SCIPs) enable targeted genomic integration of genetic payloads for rapid cell engineeringSemantic proximity Read

Miniaturized CAR knocked onto CD3ε extends TCR function with CAR specificity under control of endogenous TCR signaling cascadeSemantic proximity Read

The transformative potential of AI-driven CRISPR-Cas9 genome editing to enhance CAR T-cell therapySemantic proximity Read

An aptamer-mediated base editing platform for simultaneous knock-in and multiple gene knockout for allogeneic CAR-T cells generationSemantic proximity Read

An evolved AAV variant enables efficient genetic engineering of murine T cellsSemantic proximity Read