Site-specific replacement of large-scale DNA fragments in human cells

Abstract excerpt

Despite advances in genome editing 1-4 , precisely replacing large-scale fragments in human cells remains a significant challenge. Here, we present a site-specific gene replacement tool, named Prime Assembly (PA), which adapts prime editors to produce one or two pairs of 3’-flaps on both the genome and donor plasmids. These 3’-flaps anneal to each other precisely, similar to “Gibson Assembly” in DNA oligonucleoti...

Topics

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• beta Catenin
• Copper-Transporting ATPases
• CRISPR-Cas Systems
• Deoxyribonuclease I
• Gene Editing
• Mutation
• Point Mutation
• RNA, Guide, CRISPR-Cas Systems

Identifiers and source

Literature Corpus work

79f9404e-3a4b-5cc7-8b7e-72178f793150

DOI

10.1101/2025.09.14.676134