Article

Measuring pH in insulin secretory granules using phasor fluorescence lifetime imaging of a genetically encoded sensor

2024-10-18

Abstract excerpt

<title>Abstract</title> <p>It is widely accepted that the pH of insulin granules is acidic, and that its active regulation during granule maturation plays a role in the process of insulin secretion by β-cells. Yet, a calibrated measurement of the absolute granule pH with organelle specificity is still lacking. To tackle this issue, we used the genetically encoded E<sup>1</sup>GFP pH reporter inserted into the C-p...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 7c080687-ca31-5824-8c76-8bca436350fa
DOI: 10.21203/rs.3.rs-4968583/v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Measuring pH in insulin secretory granules using phasor fluorescence lifetime imaging of a genetically encoded sensorDOI 10.21203/rs.3.rs-4968583/v1Read

It's green outside: tracking cell surface proteins with pH-sensitive GFP.Semantic proximity Read

A superfolder variant of pH-sensitive pHluorin for in vivo pH measurements in the endoplasmic reticulum.Semantic proximity Read

A genetically encoded, pH-sensitive mTFP1 biosensor for probing lysosomal pHSemantic proximity Read

pHLARE: A Genetically Encoded Ratiometric Lysosome pH BiosensorSemantic proximity Read

Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins.Semantic proximity Read

Litmus-Body: a Molecularly Targeted Sensor for Cell-Surface pH MeasurementsSemantic proximity Read

A genetically-encoded YFP sensor with enhanced chloride sensitivity, photostability and reduced ph interference demonstrates augmented transmembrane chloride movement by gerbil prestin (SLC26a5).Semantic proximity Read

Resolving subcellular pH with a quantitative fluorescent lifetime biosensorSemantic proximity Read

pHmScarlet is a pH-sensitive red fluorescent protein to monitor exocytosis docking and fusion steps.Semantic proximity Read

Requirement of multiple protein domains and residues for gating K(ATP) channels by intracellular pH.Semantic proximity Read

SCO-ph: Microfluidic Dynamic Phenotyping Platform for High-Throughput Screening of Single Cell Acidification.Semantic proximity Read

Simultaneous intracellular chloride and pH measurements using a GFP-based sensor.Semantic proximity Read