Article
A genetically-encoded YFP sensor with enhanced chloride sensitivity, photostability and reduced ph interference demonstrates augmented transmembrane chloride movement by gerbil prestin (SLC26a5).
PloS one - 1 Jan 2014
Zhong Sheng, Navaratnam Dhasakumar, Santos-Sacchi Joseph
Abstract excerpt
BACKGROUND: Chloride is the major anion in cells, with many diseases arising from disordered Cl- regulation. For the non-invasive investigation of Cl- flux, YFP-H148Q and its derivatives chameleon and Cl-Sensor previously were introduced as genetically encoded chloride indicators. Neither the Cl- sensitivity nor the pH-susceptibility of these modifications to YFP is optimal for precise measurements of Cl- under...
Topics
- Animals
- Anion Transport Proteins
- Anions
- Bacterial Proteins
- Chlorides
- Gerbillinae
- Hydrogen-Ion Concentration
- Light
- Luminescent Proteins
- Mutation
- Photobleaching