Article
It's green outside: tracking cell surface proteins with pH-sensitive GFP.
Trends in neurosciences - 1 May 2004
Ashby Michael C, Ibaraki Kyoko, Henley Jeremy M
Abstract excerpt
Green fluorescent protein (GFP) and mutated GFP variants have proved to be immensely powerful tools that have had a profound impact on research in biological sciences. This review considers the development, use and future implications of pH-dependent GFP variants (e.g. pHluorins). These proteins hold considerable promise for the relatively non-invasive monitoring of events such as exocytosis, endocytosis and...
Topics
- Animals
- Cell Physiological Phenomena
- Green Fluorescent Proteins
- Hydrogen-Ion Concentration
- Luminescent Proteins
- Membrane Proteins
- Mutation
- Protein Transport