Article
pHmScarlet is a pH-sensitive red fluorescent protein to monitor exocytosis docking and fusion steps.
Nature communications - 3 Mar 2021
Liu Anyuan, Huang Xiaoshuai, He Wenting, Xue Fudong, Yang Yanrui, Liu Jiajia, Chen Liangyi, Yuan Lin, Xu Pingyong
Abstract excerpt
pH-sensitive fluorescent proteins (FPs) are highly advantageous for the non-invasive monitoring of exocytosis events. Superecliptic pHluorin (SEP), a green pH-sensitive FP, has been widely used for imaging single-vesicle exocytosis. However, the docking step cannot be visualized using this FP, since the fluorescence signal inside vesicles is too low to be observed during docking process. Among the available red...
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