Article
Requirement of multiple protein domains and residues for gating K(ATP) channels by intracellular pH.
The Journal of biological chemistry - 28 Sept 2001
Piao H, Cui N, Xu H, Mao J, Rojas A, Wang R, Abdulkadir L, Li L, Wu J, Jiang C
Abstract excerpt
ATP-sensitive K(+) channels (K(ATP)) are regulated by pH in addition to ATP, ADP, and phospholipids. In the study we found evidence for the molecular basis of gating the cloned K(ATP) by intracellular protons. Systematic constructions of chimerical Kir6.2-Kir1.1 channels indicated that full pH sensitivity required the N terminus, C terminus, and M2 region. Three amino acid residues were identified in these...
Topics
- Adenosine Triphosphate
- Amino Acid Sequence
- Animals
- Cloning, Molecular
- Electrophysiology
- Hydrogen-Ion Concentration
- Mice
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Oocytes
- Potassium Channels
- Potassium Channels, Inwardly Rectifying