Article
A superfolder variant of pH-sensitive pHluorin for in vivo pH measurements in the endoplasmic reticulum.
Scientific reports - 10 Aug 2018
Reifenrath Mara, Boles Eckhard
Abstract excerpt
Many cellular processes are regulated via pH, and maintaining the pH of different organelles is crucial for cell survival. A pH-sensitive GFP variant, the so-called pHluorin, has proven to be a valuable tool to study the pH of the cytosol, mitochondria and other organelles in vivo. We found that the fluorescence intensity of Endoplasmic Reticulum (ER)-targeted pHluorin in the yeast Saccharomyces cerevisiae was...
Topics
- Cytosol
- Endoplasmic Reticulum
- Fluorescent Antibody Technique
- Gene Expression
- Genes, Reporter
- Genetic Variation
- Golgi Apparatus
- Green Fluorescent Proteins
- Hydrogen-Ion Concentration
- Saccharomyces cerevisiae