Article

A genetically encoded, pH-sensitive mTFP1 biosensor for probing lysosomal pH

2020-11-04

Abstract excerpt

<h4>ABSTRACT</h4> Lysosomes are important sites for macromolecular degradation, defined by an acidic lumenal pH of ∼4.5. To better understand lysosomal pH, we designed a novel, genetically encoded, fluorescent protein (FP) based pH biosensor called FIRE-pHLy ( F luorescence Indicator RE porting pH in Ly sosomes). This biosensor was targeted to lysosomes with lysosomal-associated membrane protein 1 (LAMP1) and...

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Literature Corpus work: 367a939a-7b61-57b6-8d7c-4ef772b8dab1
DOI: 10.1101/2020.11.04.368654

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