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Article

Simultaneous intracellular chloride and pH measurements using a GFP-based sensor.

Nature methods - 1 Jul 2010

Arosio Daniele, Ricci Fernanda, Marchetti Laura, Gualdani Roberta, Albertazzi Lorenzo, Beltram Fabio

Abstract excerpt

Chloride and protons perform important closely related roles in many cellular responses. Here we developed a ratiometric biosensor, ClopHensor, based on a highly chloride-sensitive Aequorea victoria GFP variant that is suited for the combined real-time optical detection of pH changes and chloride fluxes in live cells. We detected high chloride concentration in large dense-core exocytosis granules by targeting...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 20581829
DOI: 10.1038/nmeth.1471

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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