Article

Tuning Mitotic Recombination with Patterned DNA Nicks for Precision Mosaic Analysis

2025-10-31

Abstract excerpt

<h4>ABSTRACT</h4> CRISPR/Cas9-based mosaic analysis is a powerful tool for in vivo genetics but is limited by cytotoxicity and mutagenesis associated with DNA double-strand breaks (DSBs). Here, we establish Cas9-derived nickases as safer and more reliable alternatives for inducing mitotic recombination in Drosophila . We demonstrate that single-strand nicks are sufficient to generate mosaic clones and systemati...

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Literature Corpus work: 70e151c5-c0ca-532b-b632-fc55525d75cf
DOI: 10.1101/2025.10.30.685647

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