Article
Heat shock-optimized CRISPR/Cas9 system for visible clonal analysis and mutant generation in Drosophila.
G3 (Bethesda, Md.) - 10 Dec 2025
Lin Yi, Ye Xiaolei, Zeng Ling, Luo Yuting, Feng Ying, Zhang Yan
Abstract excerpt
In Drosophila genetic studies, clonal analysis such as mosaic and Mosaic Analysis with a Repressible Cell Marker has been widely used to investigate gene function. Recently, the CRISPR/Cas9 system has been established as a powerful tool for efficient mutant generation; however, its application in clonal analysis has been rarely reported. Here, we present a suite of Gal4/UAS-Cas9 binary expression systems that...
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