Article
Cas9-assisted recombineering in C. elegans: genome editing using in vivo assembly of linear DNAs.
Nucleic acids research - 6 Sept 2016
Paix Alexandre, Schmidt Helen, Seydoux Geraldine
Abstract excerpt
Recombineering, the use of endogenous homologous recombination systems to recombine DNA in vivo, is a commonly used technique for genome editing in microbes. Recombineering has not yet been developed for animals, where non-homology-based mechanisms have been thought to dominate DNA repair. Here, we demonstrate, using Caenorhabditis elegans, that linear DNAs with short homologies (∼35 bases) engage in a highly...
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