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Article

Inducing multiple nicks promotes interhomolog homologous recombination to correct heterozygous mutations in somatic cells.

Nature communications - 15 Sept 2023

Tomita Akiko, Sasanuma Hiroyuki, Owa Tomoo, Nakazawa Yuka, Shimada Mayuko, Fukuoka Takahiro, Ogi Tomoo, Nakada Shinichiro

Abstract excerpt

CRISPR/Cas9-mediated gene editing has great potential utility for treating genetic diseases. However, its therapeutic applications are limited by unintended genomic alterations arising from DNA double-strand breaks and random integration of exogenous DNA. In this study, we propose NICER, a method for correcting heterozygous mutations that employs multiple nicks (MNs) induced by Cas9 nickase and a homologous...

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Identifiers

PMID: 37714828
DOI: 10.1038/s41467-023-41048-5
PMCID: PMC10504326

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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