Article

In vitro reconstitution of minimal human centrosomes

2025-02-23

Abstract excerpt

<h4>ABSTRACT</h4> CDK5RAP2/CEP215 is a key pericentriolar material (PCM) protein that recruits microtubule-nucleating factors at human centrosomes. Using an in vitro reconstitution system, we show that CDK5RAP2 is sufficient to form micron-scale scaffolds around a nanometer-scale nucleator in a PLK-1-regulated manner. CDK5RAP2 assemblies recruited and activated gamma tubulin ring complexes (γ-TuRCs) which, in th...

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Identifiers and source

Literature Corpus work: 6ee0ee28-4723-5654-9430-b77d7c6ef9b3
DOI: 10.1101/2025.02.20.639226

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