Article
The conserved Spd-2/CEP192 domain adopts a unique protein fold to promote centrosome scaffold assembly.
Science advances - 21 Mar 2025
Hu Liuyi, Wainman Alan, Andreeva Antonina, Apizi Muladili, Alvarez-Rodrigo Ines, Wong Siu-Shing, Saurya Saroj, Sheppard Devon, Cottee Matthew, Johnson Steven, Lea Susan M, Raff Jordan W, van Breugel Mark, Feng Zhe
Abstract excerpt
Centrosomes form when centrioles assemble pericentriolar material (PCM) around themselves. Spd-2/CEP192 proteins, defined by a conserved "Spd-2 domain" (SP2D) comprising two closely spaced AspM-Spd-2-Hydin (ASH) domains, play a critical role in centrosome assembly. Here, we show that the SP2D does not target Drosophila Spd-2 to centrosomes but rather promotes PCM scaffold assembly. Crystal structures of the human...
Read the complete abstract on PubMedTopics
- Centrosome
- Animals
- Humans
- Drosophila Proteins
- Protein Domains
- Crystallography, X-Ray