Article

Paired guide RNA CRISPR-Cas9 screening for protein-coding genes and lncRNAs involved in transdifferentiation of human B-cells to macrophages

2021-04-27

Abstract excerpt

<h4>ABSTRACT</h4> CRISPR-Cas9 screening libraries have arisen as a powerful tool to identify both protein coding (pc) and non-coding genes playing a role along different processes. In particular, the usage of a nuclease active Cas9 coupled to a single gRNA has proven to efficiently impair the expression of pc-genes by generating deleterious frameshifts. Here, we first demonstrate that the usage of a second gRNA t...

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Literature Corpus work: 6ecdd9ad-b634-57ab-a7e7-540457b94a9a
DOI: 10.1101/2021.04.26.441397

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