Article

Paired Guide RNA CRISPR-Cas9 Screening for Protein-Coding Genes and lncRNAs Involved in Transdifferentiation of Human B-Cells to Macrophages

2021-10-28

Abstract excerpt

CRISPR-Cas9 screening libraries have arisen as a powerful tool to identify protein coding (pc) and non-coding genes playing a role along different processes. In particular, the usage of a nuclease active Cas9 coupled to a single gRNA has proven to efficiently impair the expression of pc-genes by generating deleterious frameshifts. Here, we first demonstrate that the usage of a second gRNA targeting the same gene s...

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Literature Corpus work: 65758d8f-f4e2-5d36-a834-37d90e99a30b
DOI: 10.21203/rs.3.rs-988820/v1

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