Article

Virus-like particles enable targeted gene engineering and pooled CRISPR screening in primary human myeloid cells

2025-12-14

Abstract excerpt

<h4>ABSTRACT</h4> Primary human myeloid cells are promising candidates for immunotherapy, yet efficient and scalable technologies for genetic engineering and screening in these cells are limited. Here we present a virus-like particle (VLP)-based toolkit that delivers diverse CRISPR genome editing modalities to human monocytes, macrophages, and dendritic cells with high efficiency while preserving viability and in...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 0c7910b5-c9e7-517b-aa09-b63a692445f8
DOI: 10.64898/2025.12.14.692434

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Virus-like particles enable targeted gene engineering and pooled CRISPR screening in primary human myeloid cellsDOI 10.64898/2025.12.14.692434Read

A vesicle-based platform for high-efficiency, high-viability CRISPR/Cas9 knockout in primary human myeloid cellsSemantic proximity Read

Efficient Generation of Isogenic Primary Human Myeloid Cells using CRISPR-Cas9 RibonucleoproteinsSemantic proximity Read

Hybrid ssDNA repair templates enable high yield genome engineering in primary cells for disease modeling and cell therapy manufacturingSemantic proximity Read

A CRISPR-Cas9 Genome Engineering Platform in Primary CD4+ T Cells for the Interrogation of HIV Host FactorsSemantic proximity Read

Protocol for Non-viral HDR-based CRISPR/Cas9 platform for small custom editing in primary T cellsSemantic proximity Read

High throughput CRISPR screening identifies genes involved in macrophage viability and inflammatory pathwaysSemantic proximity Read

Generation of knock-in primary human T cells using Cas9 ribonucleoproteinsSemantic proximity Read

Efficient gene knockout in primary human and murine myeloid cells by non-viral delivery of CRISPR-Cas9.Semantic proximity Read

High-yield genome engineering in primary cells using a hybrid ssDNA repair template and small-molecule cocktails.Semantic proximity Read

An efficient lentiviral CRISPRi approach to silence genes in primary human monocytesSemantic proximity Read

Enhancing precision in cancer treatment: the role of gene therapy and immune modulation in oncologySemantic proximity Read

Genome-wide CRISPR Screens in Primary Human T Cells Reveal Key Regulators of Immune FunctionSemantic proximity Read