Article

Efficient Generation of Isogenic Primary Human Myeloid Cells using CRISPR-Cas9 Ribonucleoproteins

2020-03-15

Abstract excerpt

<h4>Summary</h4> Genome engineering of primary human cells with CRISPR-Cas9 has revolutionized experimental and therapeutic approaches to cell biology, but human myeloid-lineage cells have remained largely genetically intractable. We present a method for delivery of CRISPR-Cas9 ribonucleoprotein (RNP) complexes by nucleofection directly into CD14+ human monocytes purified from peripheral blood, leading to high ra...

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Literature Corpus work: 155b5c61-507f-5327-a0ea-f220f26e37c6
DOI: 10.1101/2020.03.13.991414

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