Article

CROP-Seq: a single-cell CRISPRi platform for characterizing candidate genes relevant to metabolic disorders in human adipocytes

2022-06-27

Abstract excerpt

<h4>Objective</h4> CROP-Seq combines gene silencing using CRISPR interference (CRISPRi) with single-cell RNA sequencing (scRNA-Seq) to conduct a functional reverse genetic screen of novel gene targets associated with adipocyte differentiation or function, with single-cell transcriptomes as the readout. <h4>Methods</h4> We created a human preadipocyte SGBS cell line with stable expression of KRAB-dCas9 for CRISPR...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 915e1e4c-0e28-5899-a3a9-4b7a3721457b
DOI: 10.1101/2022.06.27.497796

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

CROP-Seq: a single-cell CRISPRi platform for characterizing candidate genes relevant to metabolic disorders in human adipocytesDOI 10.1101/2022.06.27.497796Read

Bidirectional manipulation of gene expression in adipocytes using CRISPRa and siRNASemantic proximity Read

A single-cell CRISPRi platform for characterizing candidate genes relevant to metabolic disorders in human adipocytesSemantic proximity Read

CRISPR/Cas9-mediated gene knockout in human adipose stem/progenitor cells.Semantic proximity Read

CRISPR-enhanced human adipocyte “browning” as cell therapy for metabolic diseaseSemantic proximity Read

CRISPR/Cas9-mediated deletion of adipocyte genes associated with NAFLD alters adipocyte lipid handling and reduces steatosis in hepatocytes in vitroSemantic proximity Read

<i>Single-cell transcriptome dataset of human and mouse</i> in vitro <i>adipogenesis models</i>Semantic proximity Read

CRISPR delivery particles for developing therapeutic strategies in metabolic diseaseSemantic proximity Read

Proof-of-concept for CRISPR/Cas9 gene editing in human preadipocytes: Deletion of FKBP5 and PPARG and effects on adipocyte differentiation and metabolismSemantic proximity Read

A functional genetic toolbox for human tissue-derived organoidsSemantic proximity Read

Designer Fat Cells: Adipogenic Differentiation of CRISPR-Cas9 Genome-Engineered Induced Pluripotent Stem CellsSemantic proximity Read

RNA-guided cell targeting with CRISPR/RfxCas13d collateral activity in human cellsSemantic proximity Read

Combining evidence from human genetic and functional screens to identify pathways altering obesity and fat distributionSemantic proximity Read