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Mutation Knock-in Methods Using Single-Stranded DNA and Gene Editing Tools in Zebrafish.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2024

Prykhozhij Sergey V, Berman Jason N

Abstract excerpt

Introduction or knock-in of precise genomic modifications remains one of the most important applications of CRISPR/Cas9 in all model systems including zebrafish. The most widely used type of donor template containing the desired modification is single-stranded DNA (ssDNA), either in the form of single-stranded oligodeoxynucleotides (ssODN) (<150 nucleotides (nt)) or as long ssDNA (lssDNA) molecules (up to about...

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PMID: 37668920
DOI: 10.1007/978-1-0716-3401-1_19

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