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Article

CRISPR/Cas9-mediated precise genome modification by a long ssDNA template in zebrafish.

BMC genomics - 21 Jan 2020

Bai Haipeng, Liu Lijun, An Ke, Lu Xiaochan, Harrison Michael, Zhao Yanqiu, Yan Ruibin, Lu Zhijie, Li Song, Lin Shuo, Liang Fang, Qin Wei

Abstract excerpt

BACKGROUND: Gene targeting by homology-directed repair (HDR) can precisely edit the genome and is a versatile tool for biomedical research. However, the efficiency of HDR-based modification is still low in many model organisms including zebrafish. Recently, long single-stranded DNA (lssDNA) molecules have been developed as efficient alternative donor templates to mediate HDR for the generation of conditional...

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Identifiers

PMID: 31964350
DOI: 10.1186/s12864-020-6493-4
PMCID: PMC6974980

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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