Article

Strand Displacement-Driven CRISPR/Cas12a Framework Enables Precise Identification of Low-Abundance Point Mutations

2025-03-27

Abstract excerpt

Accurate identification of low-abundance point mutations is a crucial technical challenge in clinical diagnosis, particularly within high wild-type genomic backgrounds. We established an engineered CRISPR/Cas12a point mutation detection strategy (E-CRISPR) for low-abundance point mutations detection. By integrating the precise regulatory mechanism of strand displacement reaction (SDR) with the local high-concentra...

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Literature Corpus work: 65fa0aa2-dbd9-5039-bbe4-b79029203b6f
DOI: 10.22541/au.174308275.57535141/v1

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