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A rapid and ultrasensitive CRISPR-Cas12a assay for clinical detection of pathogens and mutations.

Analytical and bioanalytical chemistry - 1 Apr 2026

Fu Tao, Zhao Dongdong, Wang Qin, Peng Nanyue, Zhang Le, Chen Siqi, Ren Jiangbin, Tang Fan, Pu Ke, Wu Qiaoli, Bi Changhao, Zhang Xueli, Li Qingguo

Abstract excerpt

CRISPR-Cas12a-driven nucleic acid diagnostics offer considerable potential for highly specific and rapid detection. However, their practical applications are limited by the necessity for pre-amplification of target DNA to enhance sensitivity. To overcome this limitation, we developed Auto-catalyst, a novel one-pot, amplification-free DNA detection platform employing a two-stage autocatalytic Cas12a cascade. This...

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PMID: 41652143
DOI: 10.1007/s00216-026-06373-w

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IDH1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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