A rapid and ultrasensitive CRISPR-Cas12a assay for clinical detection of pathogens and mutations.

doi:10.1007/s00216-026-06373-w

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Article

A rapid and ultrasensitive CRISPR-Cas12a assay for clinical detection of pathogens and mutations.

Analytical and bioanalytical chemistry - 1 Apr 2026

Fu Tao, Zhao Dongdong, Wang Qin, Peng Nanyue, Zhang Le, Chen Siqi, Ren Jiangbin, Tang Fan, Pu Ke, Wu Qiaoli, Bi Changhao, Zhang Xueli, Li Qingguo

Abstract excerpt

CRISPR-Cas12a-driven nucleic acid diagnostics offer considerable potential for highly specific and rapid detection. However, their practical applications are limited by the necessity for pre-amplification of target DNA to enhance sensitivity. To overcome this limitation, we developed Auto-catalys...

Topics

Humans
CRISPR-Cas Systems
Isocitrate Dehydrogenase
Mutation
Limit of Detection
Glioma
Rapid Diagnostic Tests
Brain Neoplasms
Nucleic Acid Amplification Techniques
Bacterial Proteins
Endodeoxyribonucleases
CRISPR-Associated Proteins

Source

Open in PubMed

Identifiers

PMID: 41652143
DOI: 10.1007/s00216-026-06373-w

Genes

IDH1

Variants

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-41652143-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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