FOCUS: A Dual-Mismatch crRNA Strategy Unlocks High-Fidelity One-Step SNV Detection with Cas12a.

Abstract excerpt

CRISPR/Cas12a has emerged as a powerful tool for nucleic acid detection; however, its clinical utility is severely hampered by intrinsic limitations in single-nucleotide variant (SNV) discrimination, reliance on pre-processed single-stranded DNA (ssDNA) templates, and cumbersome multi-step workflows. Here, we report a novel molecular design principle by engineering crRNAs with site-specific dual mismatches...

Topics

• CRISPR-Cas Systems
• Polymorphism, Single Nucleotide
• CRISPR-Associated Proteins
• Humans
• Endodeoxyribonucleases
• Bacterial Proteins