Article
CASMART, a one-step CRISPR Cas12a-mediated isothermal amplification for rapid and high-resolution digital detection of rare mutant alleles.
Biosensors & bioelectronics - 15 Feb 2023
Zhang Chanqiong, Cai Zhengyi, Zhou Zihao, Li Mei, Hong Weilong, Zhou Wenxian, Yu Dianjun, Wei Panpan, He Jialin, Wang Yujuan, Huang Chongan, Wang Xiaobing, Wu Jinyu
Abstract excerpt
Convenient, ultrasensitive, and accurate detection of rare variants is essential for early cancer diagnosis and precision medicine, however, despite years of efforts, tools that have all these qualities remain elusive. Here, we developed a one-step CRISPR/Cas12a-based digital diagnostic platform for accurately quantifying mutant alleles, referred to as the CRISPR ASsoaciated Mutation Allele Rapid Test (CASMART)....
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