Article
A universal light-controlled highly sensitive one-pot CRISPR/Cas12a diagnostic based on structure-engineered crRNA.
Trends in biotechnology - 1 Sept 2026
Cui Jieyu, Zhang Lulu, Zhou Jing, Shi Ting, Wu Shangyi, Dai Tenghui, Hao Lijun, Pan Junjie, Lai Xiaodan, Lu Wentao, Huang Xingxu, Li Zhanjun, Lai Liangxue, Wang Xinjie
Abstract excerpt
Clustered regularly interspaced short palindromic repeats (CRISPR)-based nucleic acid detection has transformed molecular diagnostics through its speed and accuracy; however, one-pot formats are often limited by sensitivity and field suitability. Herein, we developed a universal light-controlled high-sensitivity one-pot CRISPR/Cas12a testing (ULTRAt) platform based on structure-engineered CRISPR RNA (crRNA)...
Read the complete abstract on PubMedTopics
- CRISPR-Cas Systems
- Humans
- Clustered Regularly Interspaced Short Palindromic Repeats
- Nucleic Acid Amplification Techniques
- Human papillomavirus 16