Article

Pervasive translation of the downstream ORF from bicistronic mRNAs by human cells: impact of the upstream ORF codon usage and splicing

2024-04-26

Abstract excerpt

In this work, we present a straightforward model to study gene expression regulation from virus-like bicistronic mRNAs in human cells, composed by a short shble upstream ORF and a reporter egfp downstream ORF. We have engineered thirteen synonymous versions of the shble ORF to explore a large parameter space in compositional and folding features, as well as differences in propensity to undergo splicing. Our exp...

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Literature Corpus work: 82a9a980-e360-5e9b-bbe3-e2db6b0b45dc
DOI: 10.1101/2024.04.26.591296

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