Article

A comprehensive view of somatic mosaicism by single-cell DNA analysis

2025-11-03

Abstract excerpt

Single-cell DNA sequencing offers a powerful means of studying somatic mosaicism but requires careful analysis to mitigate DNA amplification-related artifacts. We performed primary template-directed amplification (PTA) and sequencing of 102 nuclei from postmortem lung and colon tissues of a 74-year-old male. Single-cell mutation burdens and spectra were validated by duplex sequencing and revealed heterogeneity acr...

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Literature Corpus work: 63685deb-4522-5fe4-805f-68f606bd9d6a
DOI: 10.1101/2025.10.31.685648

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