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Analyzing somatic mutations by single-cell whole-genome sequencing.

Nature protocols - 1 Feb 2024

Zhang Lei, Lee Moonsook, Maslov Alexander Y, Montagna Cristina, Vijg Jan, Dong Xiao

Abstract excerpt

Somatic mutations are the cause of cancer and have been implicated in other, noncancerous diseases and aging. While clonally expanded mutations can be studied by deep sequencing of bulk DNA, very few somatic mutations expand clonally, and most are unique to each cell. We describe a detailed protocol for single-cell whole-genome sequencing to discover and analyze somatic mutations in tissues and organs. The...

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High-Throughput Nucleotide Sequencing
Mutagenesis
Mutation
Nucleotides
Sequence Analysis, DNA

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Whole Genome Sequencing

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PMID: 37996541
DOI: 10.1038/s41596-023-00914-8
PMCID: PMC11406548

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