Article
Protocol for genome-wide analysis of somatic variants at single-cell resolution using primary template-directed DNA amplification.
STAR protocols - 21 Mar 2025
Derks Lucca L M, van Leeuwen Anaïs J C N, Steemers Alexander S, Trabut Laurianne, van Roosmalen Markus J, Poort Vera M, Hagelaar Rico, Verheul Mark, Middelkamp Sjors, van Boxtel Ruben
Abstract excerpt
The study of somatic mutations in single cells provides insights into aging and carcinogenesis, which is complicated by the dependency on whole-genome amplification (WGA). Here, we describe a detailed workflow starting from single-cell isolation to WGA by primary template-directed amplification (PTA), sequencing, quality control, and downstream analyses. A machine learning approach, the PTA Analysis Toolkit...
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