CRISPR-based dissection of miRNA binding sites using isogenic cell lines is hampered by pervasive noise.

Abstract excerpt

Non-coding regulatory sequences play essential roles in adjusting gene output to cellular needs and are thus critical to animal development and health. Numerous such sequences have been identified in mammalian genomes ranging from transcription factors binding motifs to recognition sites for RNA-binding proteins and non-coding RNAs. The advent of CRISPR has raised the possibility of assigning functionality to...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Binding Sites
• Share to Cell Line
• Share to Clustered Regularly Interspaced Short Palindromic Repeats