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Article

A CRISPR/Cas-Mediated Selection-free Knockin Strategy in Human Embryonic Stem Cells.

Stem cell reports - 9 Jun 2015

Zhu Zengrong, Verma Nipun, González Federico, Shi Zhong-Dong, Huangfu Danwei

Abstract excerpt

The development of new gene-editing tools, in particular the CRISPR/Cas system, has greatly facilitated site-specific mutagenesis in human embryonic stem cells (hESCs), including the introduction or correction of patient-specific mutations for disease modeling. However, integration of a reporter gene into an endogenous locus in hESCs still requires a lengthy and laborious two-step strategy that involves first...

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PMID: 26028531
DOI: 10.1016/j.stemcr.2015.04.016
PMCID: PMC4471821

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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