Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Comprehensive Protocols for CRISPR/Cas9-based Gene Editing in Human Pluripotent Stem Cells.

Current protocols in stem cell biology - 17 Aug 2016

Santos David P, Kiskinis Evangelos, Eggan Kevin, Merkle Florian T

Abstract excerpt

Genome editing of human pluripotent stem cells (hPSCs) with the CRISPR/Cas9 system has the potential to revolutionize hPSC-based disease modeling, drug screening, and transplantation therapy. Here, we aim to provide a single resource to enable groups, even those with limited experience with hPSC culture or the CRISPR/Cas9 system, to successfully perform genome editing. The methods are presented in detail and are...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cell Culture Techniques
Share to Colony-Forming Units Assay

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Gene Editing

Share to Gene Knockout Techniques

Share to Gene Targeting

Share to Oligonucleotides

Share to Pluripotent Stem Cells

Share to RNA, Guide, CRISPR-Cas Systems

Source

Open in PubMed Open full text

Identifiers

PMID: 27532820
DOI: 10.1002/cpsc.15
PMCID: PMC4988528

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Comprehensive Protocols for CRISPR/Cas9-based Gene Editing in Human Pluripotent Stem Cells.PMID 27532820Read ↗

Genome Editing in Human Pluripotent Stem Cells: Approaches, Pitfalls, and Solutions.Semantic proximity Read ↗

CRISPR‐Cas9‐Based Genome Editing of Human Induced Pluripotent Stem CellsSemantic proximity Read ↗

Genome-Scale CRISPR Screening Identifies Novel Human Pluripotent Gene NetworksSemantic proximity Read ↗

Semi-automated optimized method to isolate CRISPR/Cas9 edited human pluripotent stem cell clones.Semantic proximity Read ↗

Toward a CRISPR Picture: Use of CRISPR/Cas9 to Model Diseases in Human Stem Cells In Vitro.Semantic proximity Read ↗

Genome editing of human pluripotent stem cells to generate human cellular disease modelsSemantic proximity Read ↗

Genetic Modification in Human Pluripotent Stem Cells by Homologous Recombination and CRISPR/Cas9 System.Semantic proximity Read ↗

Gene editing with 'pencil' rather than 'scissors' in human pluripotent stem cells.Semantic proximity Read ↗

Crispr-mediated Gene Targeting of Human Induced Pluripotent Stem Cells.Semantic proximity Read ↗

An iCRISPR platform for rapid, multiplexable, and inducible genome editing in human pluripotent stem cells.Semantic proximity Read ↗

A Method for Genome Editing in Human Pluripotent Stem Cells.Semantic proximity Read ↗

CRISPR-Cas9: a promising tool for gene editing on induced pluripotent stem cells.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.