Article

Enhanced endogenous gene tagging in human induced pluripotent stem cells via AAV6-mediated donor delivery

2024-05-25

Abstract excerpt

Systematically tagging endogenous proteins with fluorescent markers in human induced pluripotent stem cells (hiPSCs) allows observation of live cell dynamics in different cell states. However, the precise insertion of fluorescent proteins into live cells via CRISPR/Cas9-induced editing relies on homology-directed repair (HDR). The nonhomologous end-joining (NHEJ) DNA repair pathway often outcompetes HDR, resulting...

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Literature Corpus work: 4513ea12-31ca-5644-815b-2e620c65fca7
DOI: 10.1101/2024.05.24.595765

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