Article

Structure-guided engineering of type I-F CASTs for targeted gene insertion in human cells

2024-09-19

Abstract excerpt

<h4>ABSTRACT</h4> Conventional genome editing tools rely on DNA double-strand breaks (DSBs) and host recombination proteins to achieve large insertions, resulting in a heterogeneous mixture of undesirable editing outcomes. We recently leveraged a type I-F CRISPR-associated transposase (CAST) from the Pseudoalteromonas Tn 7016 transposon ( Pse CAST) for DSB-free, RNA-guided DNA integration in human cells, takin...

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Literature Corpus work: 0fa925ed-279e-507a-ab3f-44353322be91
DOI: 10.1101/2024.09.19.613948

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