Rapid, precise quantification of large DNA excisions and inversions by ddPCR

Abstract excerpt

The excision of genomic sequences using paired CRISPR-Cas nucleases is a powerful tool to study gene function, create disease models and holds promise for therapeutic gene editing. However, our understanding of the factors that favor efficient excision is limited by the lack of a rapid, accurate measurement of DNA excision outcomes that is free of amplification bias. Here, we introduce ddXR ( d roplet d igital P...

Topics

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• CRISPR and Genetic Engineering
• CRISPR-Cas Systems
• DNA Breaks, Double-Stranded
• DNA End-Joining Repair
• DNA Repair
• Gene Knockout Techniques
• Mutation
• Tumor Suppressor p53-Binding Protein 1

Identifiers and source

Literature Corpus work

4c053ff7-685e-5f8f-8880-32b4be06c820

DOI

10.1101/2020.04.13.039297