Article

GREPore-seq: A Robust Workflow to Detect Changes after Gene Editing through Long-range PCR and Nanopore Sequencing

2021-12-15

Abstract excerpt

To achieve the enormous potential of gene-editing technology in clinical therapies, both the on-target and unintended editing consequences need to be thoroughly evaluated. However, there is a lack of a comprehensive, pipelined, large-scale and economical workflow for detecting genome editing outcomes, in particular insertion or deletion of a large fragment. Here, we describe an approach for efficient and accurate...

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Literature Corpus work: ce6c618a-1e70-53fe-8b86-9ddd8ce47ba5
DOI: 10.1101/2021.12.13.472514

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