Article

A scalable strategy for high-throughput GFP tagging of endogenous human proteins

2016-05-24

Abstract excerpt

A central challenge of the post-genomic era is to comprehensively characterize the cellular role of the ∼20,000 proteins encoded in the human genome. To systematically study protein function in a native cellular background, libraries of human cell lines expressing proteins tagged with a functional sequence at their endogenous loci would be very valuable. Here, using electroporation of Cas9/sgRNA ribonucleoproteins...

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Literature Corpus work: d599b031-42e4-5497-b49f-edb5c18b1502
DOI: 10.1101/055285

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