Article

Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination system

2017-07-06

Abstract excerpt

<h4>ABSTRACT</h4> Deciphering a function of a given protein requires investigating various biological aspects. Usually, the protein of interest is expressed with a fusion tag that aids or allows subsequent analyses. Additionally, downregulation or inactivation of the studied gene enables functional studies. Development of the CRISPR/Cas9 methodology opened many possibilities but in many cases it is restricted to...

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Literature Corpus work: ab9437b1-a299-5b75-9cf2-73de8947e383
DOI: 10.1101/160101

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