Article

The method of choice to knock-in large inserts via CRISPR

2021-07-01

Abstract excerpt

CRISPR/Cas9 gene editing is a revolutionary method used to study gene function by transcript silencing, knock-out, or activation. The knock-in of DNA fragments to endogenous genes of interest is another promising approach to study molecular pathways but is technically challenging. Many approaches have been suggested, but the proof of correct integration has often been relied on less convenient validation experimen...

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Literature Corpus work: b51e494f-1537-5961-b233-dfb9ce73239f
DOI: 10.1101/2021.07.01.450700

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